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Resumen Antecedentes: En los pacientes con infarto agudo de miocardio con elevación del segmento ST (IAMCEST), el acondicionamiento isquémico puede ayudar a limitar la remodelación ventricular. Objetivos: Investigar el efecto del posacondicionamiento isquémico remoto (PAIR) en la función del ventrículo izquierdo durante la intervención coronaria percutánea primaria (ICPP) en pacientes con IAMCEST. Material y métodos: Estudio de intervención pre y posprueba con un total de 60 pacientes con IAMCEST. Los pacientes fueron divididos en dos grupos: con y sin PAIR. Resultados: En el seguimiento de seis meses se observó una diferencia significativa en la fracción de eyección del ventrículo izquierdo en pacientes con ICPP, la cual fue mayor en el grupo con PAIR en comparación con el grupo sin PAIR: 1.0 (−1.0 a 4.3) versus −1.0 (−4.0 a 1.3), p = 0.033. En la medición de seis meses, el volumen sistólico final del ventrículo izquierdo en los pacientes sin PAIR fue mayor en comparación con el grupo homólogo: 79.3 ± 30.5 mL versus 64.4 ± 21.4 mL, p = 0.032. Conclusiones: PAIR muestra efectos favorables en la función ventricular izquierda y, por lo tanto, en el futuro podría ser una estrategia cardioprotectora potencial contra la lesión por isquemia-reperfusión en pacientes con IAMCEST.
Abstract Background: Ischemic conditioning may help patients with ST-segment elevation myocardial infarction (STEMI) to limit ventricular remodeling. Objectives: To investigate the effect of remote ischemic postconditioning (RIPC) on left ventricular function during primary percutaneous coronary intervention (PPCI) in patients with STEMI. Material and methods: Pre- and post-test intervention study with a total of 60 STEMI patients. Patients were divided in two groups: with and without RIPC. Results: At 6-month follow-up evaluation, a significant difference in left ventricular ejection fraction was observed in patients who underwent PPCI, which was higher in the group with RIPC in comparison with the group without RIPC: 1.0 (−1.0 to 4.3) vs. −1.0 (−4.0 to 1.3), p = 0.033. In addition, at 6-month measurement, left ventricular end-systolic volume in patients without RIPC: was higher in comparison with their counterparts: 79.3 ± 30.5 mL versus 64.4 ± 21.4 mL, p = 0.032. Conclusions: RIPC shows favorable effects on left ventricular function and, therefore, in the future, it could be a potential cardioprotective strategy against ischemia-reperfusion injury in STEMI patients.
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Background: Heat shock factor protein 2 (HSF 2) and Ovo like transcriptional repressor 1 (Ovol1) genes are found in germ cells that control spermatogenesis. One reason is exposure to mosquito repellent drugs from allethrin. Allethrin is one of the causes of male reproductive dysfunction that affects male reproduction resulting in infertility.Methods: This study was an experimental post-test randomized control group design. Twenty-eight rats given exposure according to the experimental group were K (control), P1 (4 hours exposure), P2 (8hours exposure), and P3 (12hours exposure) for 30 days. Examination of HSF 2 and Ovol1 genes from testicular tissue using real-time PCR with a relatively quantitative calculation method. Implementation of research at animal houses and biomedical laboratories. Data analysis using one-way ANOVA with a significant level of p <0.05.Results: The results of this study found differences in the average number of expressions of the HSF2 and Ovol1 genes. The average expression of the HSF2 control group gene was 3.50, P1: 2.99, P2: 0.62, and P3: 0.49. Whereas in the Ovol1 gene control group were 1.17, P1: 0.80, P2: 0.57, and P3: 0.65. The results of statistical tests using one-way ANOVA are HSF2 (p = 0,000) and Ovol1 (p = 0.045).Conclusions: Based on the results of this study, it was concluded that there was an allethrin effect in decreasing the expression of the HSF 2 and Ovol1 genes in Wistar albino Rattus Novergicus strain.
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Background: YBX2 and JHDM2A gene is important in spermatogenesis which acts as biomarkers of infertility. Allethrin chemicals widely used in mosquito and potentially toxic drugs that can damage DNA. The purpose of this study was to decide the effect of allethrin on decreasing YBX2 and JHDM2A gene expression on spermatogenesis of male white mice.Methods: This research is an experimental post-test randomized control group design. Twenty-eight rats were given exposure according to the experimental group is K (control), P1 (exposure 4hours), P2 (exposure 8hours), and P3 (exposure 12hours) for 30 days. Examination of YBX2 and JHDM2A gene expression from testicular tissue using real-time PCR with a relatively quantitative calculation method. Research implementation at Animal House and Biomedical Laboratory. Data analysis using one way ANOVA with a significant level of p<0.05.Results: The results of this study were found mean differences amount of YBX2 and JHDM2A gene expression. The mean expression of the control group YBX2 gene is 1.1376, P1: 0.8976, P2: 0.5504, and P3: 0.4512. While in JHDM2A gene control group was 1.7033, P1: 1,7025, P2: 0.6863, and P3: 0.4077. Results of statistical tests using one-way ANOVA is YBX2 (p = 0.010) and JHDM2A (p = 0.000).Conclusions: Based on the results of this study it was concluded that there was an allethrin effect on the decrease in YBX2 and JHDM2A gene expression in the Wistar Albino Rattus novergicus strain.